Highly Efficient Photolabile Protecting Groups with Intramolecular Energy Transfer

摘要

DNA chips are widely used for genomic analysis.Currently,high-density DNA chips can be synthesized with up to several hundred thousand different spots on an area of 1 cm~2.In principle,the complete human genome can be probed with such a chip.The required high spot densities can be achieved by photolithographic in situ DNA-chip synthesis.In this method,the oligonucleotides are synthesized step by step from protected nucleoside phosphitamides using photolithography with masks or micromirror arrays as suitable means for achieving parallel spatial addressability.