Label-Free, Real-Time Glycosyltransferase Assay Based on a Fluorescent Artificial Chemosensor

摘要

Glycosyltransferases are involved in the biosynthesis of oligosaccharides, which are vital for all living systems. This family of enzymes plays a multifunctional role in cell physiology and has been associated with embryonic maturation, cell-cell recognition, cell development, and sperm-egg binding.[1] Selective inhibition of glycosyltransferase is thus of interest as it may lead to the development of new therapeutic agents.[2] However, the lack of a rapid and simple method for continuously monitoring glycosyltransferase activity prevents the efficient discovery of potent drug candidates.