Microsphere-Based Real-Time Calcium Sensing

摘要

Fluorescent sensors for a variety of ions,such as Ca~(2+) and Na~+,are among the most useful tools available for studying ion fluxes within the intracellular environment following the modulation or stimulation of a range of complex biological processes.Two widely used fluorescent indicators for Ca~(2+) ions are Indo-1 (1) and Fura-2 (2),which complex calcium with high affinity (K_d = 230 and 145 nM,respectively),and these reagents have allowed numerous investigations into intracellular free Ca~(2+) concentrations following a range of cellular insults.