A Photoinducible 1,3-Dipolar Cycloaddition Reaction for Rapid,Selective Modification of Tetrazole-Containing Proteins

摘要

Bioorthogonal chemistry provides an exciting new strategy to visualize protein expression,track protein localization,measure protein activity,and identify protein interaction partners in living systems.Two steps are typically involved in this approach:1) the incorporation of a bioorthogonal group into a protein through either a biochemical pathway or semisyn-thesis;2) a site-specific reaction between the protein that carries the bioorthogonal group and a cognate small-molecule probe.