Identification of a High-Affinity-Binding Oligo-saccharide by (+) Nanoelectrospray Quadrupole Time-of-Flight Tandem Mass Spectrometry of a Noncovalent Enzyme-Ligand Complex

摘要

Oligosaccharides are of current interest as targets for the development of novel pharmaceuticals and plant-growth regulators.For example,so-called heterochitooligosacchar-ides,which are composed of N-acetylglucosamine (GlcNAc or A) and glucosamine (GlcN or D),exhibit various biological activities,such as promotion of chondrocyte growth in cell culture,morphogenetic activity in vertebrates,and elicitor action in plants.