Identification of the C‐Glycoside Synthases during Biosynthesis of the Pyrazole‐C‐Nucleosides Formycin and Pyrazofurin

摘要

Abstract > C ‐Nucleosides are characterized by a C?C rather than a C?N linkage between the heterocyclic base and the ribofuranose ring. While the biosynthesis of pseudouridine‐ C ‐nucleosides has been studied, less is known about the pyrazole‐ C ‐nucleosides such as the formycins and pyrazofurin. Herein, genome screening of Streptomyces candidus NRRL 3601 led to the discovery of the pyrazofurin biosynthetic gene cluster pyf . In?vitro characterization of gene product PyfQ demonstrated that it is able to catalyze formation of the C ‐glycoside carboxyhydroxypyrazole ribonucleotide (CHPR) from 4‐hydroxy‐1 H ‐pyrazole‐3,5‐dicarboxylic acid and phosphoribosyl pyrophosphate (PRPP). Similarly, ForT, the PyfQ homologue in the formycin pathway, can catalyze the coupling of 4‐amino‐1 H ‐pyrazole‐3,5‐dicarboxylic acid and PRPP to form carboxyaminopyrazole ribonucleotide. Finally, PyfP and PyfT are shown to catalyze amidation of CHPR to pyrazofurin 5′‐phosphate thereby establishing the latter stages of both pyrazofurin and formycin biosynthesis. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > c </ i> - 核苷的特征在于C 2 C而不是杂环基和核尿藻糖环之间的C = N键。虽然假尿素的生物合成 - c </ i> - 已经研究过核苷，少于吡唑 - c </ i> - 核苷，如甲酰胺和吡唑脲。在此，基因组筛选链霉菌念珠菌</ i> NRRL 3601导致吡唑氏菌蛋白生物合成基因簇的发现 pyf </ i> 。在基因产物的体外表征中 pyfq </ i> 证明它能够催化形成的形成 c </ i> 糖苷羧基羟基吡唑核糖核苷酸（CHPR）来自4-羟基-1 h </ i> - 吡唑-3,5-二羧酸和磷酰基磷酸酯（PRPP）。同样，堡垒， pyfq </ i> 甲霉素途径中的同源物，可以催化4-氨基-1的偶联 h </ i> - 吡唑-3,5-二羧酸和PRPP形成羧氨基吡唑核苷酸。最后， pyfp </ i> 和 pyft </ i> 被证明催化CHPR酰胺至吡唑啉素5'-磷酸盐，从而建立吡唑脲和甲酰霉素生物合成的后一阶段。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-19011/'>《Angewandte Chemie》</a> <b style="margin: 0 2px;">|</b><span>2019年第46期</span><b style="margin: 0 2px;">|</b><span>共5页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1188.html" title="应用化学">应用化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biosynthesis&option=203" rel="nofollow">biosynthesis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biosynthetic gene cluster&option=203" rel="nofollow">biosynthetic gene cluster;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=C-glycosidation reaction&option=203" rel="nofollow">C-glycosidation reaction;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=C-nucleosides&option=203" rel="nofollow">C-nucleosides;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzyme mechanism&option=203" rel="nofollow">enzyme mechanism;</a> </p> <div class="translation"> 机译：生物合成;生物合成基因簇;C-糖苷化反应;C-核苷;酶机制; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704028165243.html">Identification of the C‐Glycoside Synthases during Biosynthesis of the Pyrazole‐C‐Nucleosides Formycin and Pyrazofurin</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> </a> <a href="/journal-foreign-19011/" target="_blank" rel="nofollow" class="tuijian_authcolor">Angewandte Chemie .</a> <span>2019</span><span>,第46期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在吡唑-C核苷酰霉霉素和吡唑氏菌素的生物合成期间鉴定C-糖苷合成酶</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704019839026.html">One‐Pot Synthesis of Aryl Pyrazole <i xmlns="http://www.wiley.com/namespaces/wiley">CC ‐Nucleoside Analogs of Pyrazofurin from Sugar Alkynes</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liu Hong&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Liu Hong,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liang Yan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Liang Yan,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jia Tong‐Tong&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Jia Tong‐Tong,</a> <a href="/journal-foreign-23259/" target="_blank" rel="nofollow" class="tuijian_authcolor">European journal of organic chemistry .</a> <span>2017</span><span>,第11期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：芳基吡唑的单壶合成<i xmlns =“http://www.wiley.com/namespaces/wiley”> c </ i>吡唑氏蛋白从糖alkynes的c-核苷类似物</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070405177535.html">Identification of the tautomeric form of formycin A in its complex with Escherichia coli purine nucleoside phosphorylase based on the effect of enzyme-ligand binding on fluorescence and phosphorescence</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wlodarczyk J&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wlodarczyk J,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Galitonov GS&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Galitonov GS,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Kierdaszuk B&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Kierdaszuk B </a> <a href="/journal-foreign-22966/" target="_blank" rel="nofollow" class="tuijian_authcolor">European Biophysics Journal .</a> <span>2004</span><span>,第5期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于酶-配体结合对荧光和磷光的影响，鉴定福霉素A与大肠杆菌嘌呤核苷磷酸化酶复合物中的互变异构形式</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-249811_thesis/070508593636.html">Emission Spectroscopy of Complex Formation between Escherichia coli Purine Nucleoside Phosphorylase (PNP) and Identified Tautomeric Species of Formycin Inhibitors Resolves Ambiguities Found in Crystallographic Studies</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=B. Kierdaszuk&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">B. Kierdaszuk </a> <a href="/conference-foreign-249811/" target="_blank" rel="nofollow" class="tuijian_authcolor">Conference on Methods and Applications of Fluorescence: Spectroscopy, Imaging, and Probes .</a> <span>2002</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：大肠杆菌嘌呤核苷磷酸酶（PNP）之间复杂形成的发射光谱谱和鉴定的甲酰霉素抑制剂的互变异构体决定了晶体研究中发现的含糊不清</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061520740.html">GENETIC AND BIOCHEMICAL STUDIES WITH CHINESE HAMSTER OVARY CELL MUTANTS RESISTANT TO THE PURINE NUCLEOSIDE ANALOGS: TOYOCAMYCIN, FORMYCIN A AND FORMYCIN B</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=MEHTA, KAMAL DEEP.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">MEHTA, KAMAL DEEP. </a> <span>1985</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：抗嘌呤核苷类似物的中国仓鼠卵巢细胞突变体的遗传和生物化学研究：TOYOCAMYCIN，FORMYCIN A和FORMYCIN B</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040006874023.html">Comparative Investigation into Formycin A and Pyrazofurin A Biosynthesis Reveals Branch Pathways for the Construction of C-Nucleoside Scaffolds</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Meng Zhang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Meng Zhang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Peichao Zhang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Peichao Zhang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gudan Xu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Gudan Xu,</a> <span>2020</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：对甲酰霉素A和Pyrozofurin的比较调查生物合成揭示了C-核苷支架构建的分支途径</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000131450115.html">Comparative Investigation into Formycin A and Pyrazofurin A Biosynthesis Reveals Branch Pathways for the Construction of C -Nucleoside Scaffolds</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Meng Zhang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Meng Zhang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Peichao Zhang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Peichao Zhang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gudan Xu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Gudan Xu,</a> <span>2020</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：对比霉素A和Pyrozofurin的比较调查生物合成揭示了C-核苷支架构建的分支途径</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_acta-academiae-medicinae-sinicae_thesis/0201254342549.html">丁酰苷菌素生物合成酶基因的分子克隆Ⅰ.重组质粒No.2和No.11的构建及其性质</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李元&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李元</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=石莲英&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,石莲英</a> <span> <a href="/journal-cn-6704/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国医学科学院学报 </a> </span> <span> . 1989</span><span>,第001期</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120101393537.html">吡唑霉素生物合成基因簇、重组菌及其应用</a> <b>[P]</b> . <span> 中国专利： CN110551739A </span> <span> . 2019-12-10</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120109067372.html">一种吡唑呋喃菌素的C‑核苷类似物及其合成方法</a> <b>[P]</b> . <span> 中国专利： CN106588891A </span> <span> . 2017-04-26</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130417738601.html">Recombinant host comprising one or more genes for the biosynthesis of steviol; isolated Nucleic Acid comprising such genes; a method for producing steviol Glycoside, steviol or a Composition of steviol Glycoside; recombinant Strain of Saccharomyces D glucoside; composition And steviol.</a> <b>[P]</b> . <span> 外国专利：  CL2012003373A1 </span> <span> . 2014-08-08</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：重组宿主，其包含一种或多种用于甜菊醇生物合成的基因;包含此类基因的分离的核酸;一种生产甜菊醇糖苷，甜菊糖或甜菊糖苷的组合物的方法;酿酒酵母D葡糖苷重组菌株;组成和甜菊醇。 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130436674845.html">New genes from biosynthesis gene clusters for the synthesis of amino glycoside - antibiotics, as well as the result of a new amino glycoside - antibiotics</a> <b>[P]</b> . <span> 外国专利：  DE102004017141A1 </span> <span> . 2005-12-01</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：来自生物合成基因簇的新基因用于合成氨基糖苷类抗生素，以及新的氨基糖苷类抗生素的结果 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130437229432.html">METHODS FOR THE IDENTIFICATION OF INHIBITORS OF ASPARAGINE SYNTHASE, 5-AMINOLEVULINATE SYNTHASE, HISTIDINOL-PHOSPHATASE, 3-ISOPROPYLMALATE AND THREONINE SYNTHASE AS ANTIBIOTICS</a> <b>[P]</b> . <span> 外国专利：  EP1461460A4 </span> <span> . 2006-07-05</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：识别天冬酰胺合成酶，5-氨基戊酸合成酶，组氨醇-磷酸酶，3-异丙酸苹果酸和苏氨酸合成酶抑制剂的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028165243','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704028165243down" data-source="7," data-out-id="wb5+pC6Ek9DiOSwjNFmyjW44VF8qCOtv0nAsoclTHdGYg0GbIofSu5LeaKUoMIIV," data-f-source-id="7" data-title="Identification of the C‐Glycoside Synthases during Biosynthesis of the Pyrazole‐C‐Nucleosides Formycin and Pyrazofurin" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="19011" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Identification of the C‐Glycoside Synthases during Biosynthesis of the Pyrazole‐C‐Nucleosides Formycin and Pyrazofurin"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.8"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.8"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>