Image‐Based Morphological Profiling Identifies a Lysosomotropic, Iron‐Sequestering Autophagy Inhibitor

摘要

Abstract > Chemical proteomics is widely applied in small‐molecule target identification. However, in general it does not identify non‐protein small‐molecule targets, and thus, alternative methods for target identification are in high demand. We report the discovery of the autophagy inhibitor autoquin and the identification of its molecular mode of action using image‐based morphological profiling in the cell painting assay. A compound‐induced fingerprint representing changes in 579 cellular parameters revealed that autoquin accumulates in lysosomes and inhibits their fusion with autophagosomes. In addition, autoquin sequesters Fe 2+ in lysosomes, resulting in an increase of lysosomal reactive oxygen species and ultimately cell death. Such a mechanism of action would have been challenging to unravel by current methods. This work demonstrates the potential of the cell painting assay to deconvolute modes of action of small molecules, warranting wider application in chemical biology. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 化学蛋白质组学广泛应用于小分子靶标鉴定。然而，一般而言，它不鉴定非蛋白质小分子靶标，因此，目标鉴定的替代方法是高需求。我们报告了通过细胞绘画测定中的基于图像的形态分析发现自噬抑制剂自动喹蛋白和其分子作用的鉴定。表示579个细胞参数变化的复合诱导的指纹显示，自身喹蛋白累积在溶酶体中并抑制其与自噬体的融合。此外，Autoquin Seatesters Fe 2 + </ sup> 在溶酶体中，导致溶酶体活性氧物质的增加和最终的细胞死亡。这种行动机制将通过当前方法解开挑战。这项工作证明了细胞绘画测定对小分子的去卷积作用模式的潜力，需要更广泛地应用化学生物学。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-19011/'>《Angewandte Chemie》</a> <b style="margin: 0 2px;">|</b><span>2020年第14期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1188.html" title="应用化学">应用化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=autophagy&option=203" rel="nofollow">autophagy;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cell painting&option=203" rel="nofollow">cell painting;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lysosome&option=203" rel="nofollow">lysosome;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=proteomics&option=203" rel="nofollow">proteomics;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=target identification&option=203" rel="nofollow">target identification;</a> </p> <div class="translation"> 机译：自噬;细胞绘画;溶酶体;蛋白质组学;目标识别; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704028161687.html">Image‐Based Morphological Profiling Identifies a Lysosomotropic, Iron‐Sequestering Autophagy Inhibitor</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> </a> <a href="/journal-foreign-19011/" target="_blank" rel="nofollow" class="tuijian_authcolor">Angewandte Chemie .</a> <span>2020</span><span>,第14期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: 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rel="nofollow" class="tuijian_auth tuijian_authcolor">Dar-Bin Shieh,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li-Xing Yang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Li-Xing Yang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wei-Ting Lee&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wei-Ting Lee,</a> <a href="/conference-foreign-205262/" target="_blank" rel="nofollow" class="tuijian_authcolor">International Conference on Nanotechnology .</a> <span>2017</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：零价铁基纳米颗粒通过线粒体介导的自噬选择性抑制癌细胞</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061520876.html">Gray-cast iron classification based on graphite flakes using image morphology and neural networks</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yeliyur Siddegowda, Darshan.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yeliyur Siddegowda, Darshan. </a> <span>2016</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于图像形态和神经网络的石墨鳞片灰铸铁分类</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040002570248.html">Identifying Small Molecules which Inhibit Autophagy: a PhenotypicScreen Using Image-Based High-Content Cell Analysis</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=J.V. Peppard&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">J.V. Peppard,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=C. Rugg&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">C. Rugg,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=M. Smicker&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">M. Smicker,</a> <span>2014</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：识别抑制自噬的小分子：表型使用基于图像的高内涵细胞分析的屏幕</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000127673472.html">Image‐Based Morphological Profiling Identifies a Lysosomotropic, Iron‐Sequestering Autophagy Inhibitor</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Luca Laraia&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Luca Laraia,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guillaume Garivet&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Guillaume Garivet,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Daniel J. Foley&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Daniel J. Foley,</a> <span>2020</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于图像的形态分析识别溶酶体溶质，铁螯合的自噬抑制剂</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_china-animal-husbandry-communication_thesis/0201296318791.html">奇力铁（蛋氨酸螯合铁、甘氨酸螯合铁）</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/journal-cn-50425/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国牧业通讯 </a> </span> <span> . 2011</span><span>,第14期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_cereal-feed-industry_thesis/0201275779428.html">蛋氨酸与铁、铜、锰、锌螯合物螯合率的测定</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨林&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杨林</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨海鹏&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨海鹏</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=钱沁&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,钱沁</a> <span> <a href="/journal-cn-9366/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 粮食与饲料工业 </a> </span> <span> . 2004</span><span>,第004期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201295452225.html">甘氨酸螯合铁、蛋氨酸螯合铜预防仔猪贫血效果的研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=许丽&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 许丽</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=韩友文&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,韩友文</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=滕冰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,滕冰</a> <span> <a href="/journal-cn-56868/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 饲料工业 </a> </span> <span> . 2001</span><span>,第11期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-sun-yat-sen-university-medical-sciences_thesis/0201246705562.html">蛋白酶体抑制剂Epoxomicin对视网膜色素上皮细胞自噬-溶酶体途径的影响</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=詹姣&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 詹姣</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=何洁儿&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,何洁儿</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=商福&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,商福</a> <span> <a href="/journal-cn-6575/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中山大学学报（医学科学版） </a> </span> <span> . 2016</span><span>,第004期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-pathophysiology_thesis/0201223292555.html">自噬溶酶体抑制剂氯化铵促进维生素K3诱导人宫颈癌HeLa细胞凋亡的作用</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=于春艳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 于春艳</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘希&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘希</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张钰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张钰</a> <span> <a href="/journal-cn-5784/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国病理生理杂志 </a> </span> <span> . 2013</span><span>,第007期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-15400_thesis/020221394268.html">氨基酸螯合铁对早期断奶仔猪内脏铁含量及铁消化率的影响</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王明镇&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王明镇</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘孟洲&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘孟洲</a> <span> <a href="/conference-cn-15400/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国西部第八届实验动物管理与学术研讨会暨四川省第二届实验动物学术年会 </a> <span> <span> . 2009</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315602038.html">Meta分析评价高血清铁蛋白及铁螯合疗法对AML和MDS 患者的预后影响</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨园&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杨园</a> <span> . 2020</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120105728958.html">HDAC抑制剂和可螯合的金属化合物的药物组合物以及金属－HDAC抑制剂螯合物</a> <b>[P]</b> . <span> 中国专利： CN101528212A </span> <span> . 2009-09-09</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120103242072.html">一种南极磷虾铁螯合肽及其铁螯合物的制备方法与应用</a> <b>[P]</b> . <span> 中国专利： CN110731512A </span> <span> . 2020-01-31</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130425481932.html">use of a compound selected from the group consisting of a proteasome inhibitor, an autophagy inhibitor, a lysosomal inhibitor, an golgi er protein transport inhibitor, an hsp90 chaperonin inhibitor, a heat shock response activator, a and a histone deacetylase inhibitor, use of 11-cis-retinal or 9-cis-retinal and a compound selected from the group consisting of a proteasome inhibitor, an autophagy inhibitor, a lissosomal inhibitor, a er to golgi protein, a hsp90 chaperonin inhibitor, a heat shock response activator, a glycosidase inhibitor, and a histone deacetylase inhibitor, a method for increasing the amount of a biochemically functional conformation of a protein in a cell, composition pharmaceutical for the treatment of an ocular pcd, pharmaceutical composition for the treatment of retinitis pigmentosa, kit for the treatment of an ocular pcd, kit for the treatment retinitis pigmentosa, method for identifying a compound useful for the treatment of an individual having an ocular pcd,</a> <b>[P]</b> . <span> 外国专利：  BRPI0615962A2 </span> <span> . 2011-05-31</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用选自以下的化合物：蛋白酶体抑制剂，自噬抑制剂，溶酶体抑制剂，高尔基体蛋白转运抑制剂，hsp90伴侣蛋白抑制剂，热休克反应激活剂，α和组蛋白脱乙酰酶抑制剂，使用11 -顺式-视黄醛或9-顺式-视黄醛以及选自蛋白酶体抑制剂，自噬抑制剂，核糖体抑制剂，高尔基体蛋白er，hsp90伴侣蛋白抑制剂，热休克反应激活剂，糖苷酶的化合物抑制剂和组蛋白脱乙酰基酶抑制剂，一种增加细胞中蛋白质的生化功能构象量的方法，用于治疗眼pcd的组合物药物，用于治疗色素性视网膜炎的药物组合物，用于治疗视网膜色素变性的试剂盒眼用pcd，用于治疗色素性视网膜炎的试剂盒，用于鉴定可用于治疗具有眼用pcd的个体的化合物的方法， </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130417399126.html">COMBINATION OF LYSOSOMOTROPIC OR AUTOPHAGY MODULATING AGENTS AND A GSK-3 INHIBITOR FOR TREATMENT OF CANCER</a> <b>[P]</b> . <span> 外国专利：  WO2013182519A1 </span> <span> . 2013-12-12</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：溶溶体或自噬调节剂与GSK-3抑制剂的组合治疗癌症 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130411939576.html">PHARMACEUTICAL COMPOSITIONS COMPRISING INHIBITORS OF IRON TRANSPORT, AND METHOD OF IDENTIFYING IRON TRANSPORT INHIBITORS, IN STAPHYLOCOCCUS AUREUS</a> <b>[P]</b> . <span> 外国专利：  CA2576247C </span> <span> . 2016-08-30</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：包含铁运输抑制剂的药物成分，以及鉴定金黄色葡萄球菌中铁运输抑制剂的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028161687','4',7,2,1,'',this,24)" class="delivery" prompt="010401" 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