Mirror‐Image 5S Ribonucleoprotein Complexes

Ling Jun‐Jie; Fan Chuyao; Qin Hong; Wang Min; Chen Ji; Wittung‐Stafshede Pernilla; Zhu Ting F.

摘要

Abstract > After realizing mirror‐image genetic replication, transcription, and reverse transcription, the biggest challenge in establishing a mirror‐image version of the central dogma is to build a mirror‐image ribosome‐based translation machine. Here, we chemically synthesized the natural and mirror‐image versions of three ribosomal proteins (L5, L18, and L25) in the large subunit of the Escherichia coli ribosome with post‐translational modifications. We show that the synthetic mirror‐image proteins can fold in?vitro despite limited efficiency and assemble with enzymatically transcribed mirror‐image 5S ribosomal RNA into ribonucleoprotein complexes. In addition, the RNA–protein interactions are chiral‐specific in that the mirror‐image ribosomal proteins do not bind with natural 5S ribosomal RNA and vice versa. The synthesis and assembly of mirror‐image 5S ribonucleoprotein complexes are important steps towards building a functional mirror‐image ribosome. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 在实现镜像遗传复制，转录和逆转录后，建立中央教条的镜像版本的最大挑战是建立一个基于镜像核糖体的平移机。在这里，我们在大亚基中化学合成了三种核糖体蛋白（L5，L18和L25）的自然和镜像版本大肠杆菌</ i> 核糖体具有翻译后修饰。我们表明，尽管效率有限并将酶促转录的镜子 - 图像5S核糖体RNA与核糖核蛋白复合物组装成核糖体RNA，但是合成镜图像蛋白质可以折叠在β体外。此外，RNA-蛋白质相互作用是手性特异性，因为镜像核糖体蛋白与天然5S核糖体RNA没有结合，反之亦然。镜像5S核糖核酸核糖蛋白复合物的合成和组装是建立功能镜像核糖体的重要步骤。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-19011/'>《Angewandte Chemie》</a> <b style="margin: 0 2px;">|</b><span>2020年第9期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ling Jun‐Jie&option=202" target="_blank" rel="nofollow">Ling Jun‐Jie;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fan Chuyao&option=202" target="_blank" rel="nofollow">Fan Chuyao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Qin Hong&option=202" target="_blank" rel="nofollow">Qin Hong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Min&option=202" target="_blank" rel="nofollow">Wang Min;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chen Ji&option=202" target="_blank" rel="nofollow">Chen Ji;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wittung‐Stafshede Pernilla&option=202" target="_blank" rel="nofollow">Wittung‐Stafshede Pernilla;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhu Ting F.&option=202" target="_blank" rel="nofollow">Zhu Ting F.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>School of Life SciencesMinistry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyBeijing 100084 China;</p> <p>School of Life SciencesMinistry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyBeijing 100084 China;</p> <p>School of Life SciencesMinistry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyBeijing 100084 China;</p> <p>School of Life SciencesMinistry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyBeijing 100084 China;</p> <p>School of Life SciencesMinistry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyBeijing 100084 China;</p> <p>Chemical Biology DivisionChalmers University of Technology41296 Gothenburg Sweden;</p> <p>School of Life SciencesMinistry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyBeijing 100084 China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1188.html" title="应用化学">应用化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chemical protein synthesis&option=203" rel="nofollow">chemical protein synthesis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chirality&option=203" rel="nofollow">chirality;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=native chemical ligation&option=203" rel="nofollow">native chemical ligation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ribonucleoproteins&option=203" rel="nofollow">ribonucleoproteins;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=solid-phase peptide synthesis&option=203" rel="nofollow">solid-phase peptide synthesis;</a> </p> <div class="translation"> 机译：化学蛋白质合成;手性;本机化学结扎;核糖核蛋白;固相肽合成; 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李忠华&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李忠华</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=郑世军&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,郑世军</a> <span> <a href="/conference-cn-8411/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国畜牧兽医学会兽医公共卫生学分会第二次全国会员代表大会暨第三次学术研讨会 </a> <span> <span> . 2012</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020316372808.html">小核核糖核蛋白E在肝细胞肝癌中的作用及机制研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘攀&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘攀</a> <span> . 2021</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120103497891.html">修饰的指导RNA，CRISPR-核糖核蛋白复合物和使用方法</a> <b>[P]</b> . <span> 中国专利： CN110753757A </span> <span> . 2020-02-04</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120103319124.html">一种可实现肿瘤靶向递送核糖核蛋白复合物的纳米粒子的制备方法和应用</a> <b>[P]</b> . <span> 中国专利： CN110638789A </span> <span> . 2020-01-03</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130401321995.html">ISOLATED MODIFIED CAS9 MOLECULE, METHOD TO PRODUCE A MODIFIED CAS9, PROTEIN OR RIBONUCLEOPROTEIN COMPLEXES OR COMPLEXES OF MIXED OR LIPID, COMPOSITE, LIPID, FUSION PROTEIN, NUTRITION, NUTRITION, NUTRITION IN VITRO USE OF A MODIFIED RECOMBINANT CAS9, PARTS KIT, AND, IN VITRO METHOD TO CHANGE A CELL GENOME</a> <b>[P]</b> . <span> 外国专利：  BR112019016765A2 </span> <span> . 2020-05-26</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：分离的改良CAS9分子，生产改良CAS9的方法，蛋白质或核糖蛋白复合物或混合或脂类，复合物，脂质，融合蛋白，营养，营养，营养的蛋白质，或在体外使用部分改良的K糖浆配制的蛋白质改变细胞基因组的体外方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130500037207.html">RNA GUIDE GENOME EDITING IN CITRUS USING CRISPR-RIBONUCLEOPROTEIN COMPLEXES</a> <b>[P]</b> . <span> 外国专利：  WO2021026081A3 </span> <span> . 2021-05-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：使用CRISPR-Ribon核蛋白复合物在柑橘中进行RNA指导基因组 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130400774652.html">Modified Guide RNAs, CRISPR-ribonucleoprotein Complexes and Methods of Use</a> <b>[P]</b> . <span> 外国专利：  KR20200017485A </span> <span> . 2020-02-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：修饰的指导RNA，CRISPR-核糖核蛋白复合物和使用方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028161501','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 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