Nanoassembly of Oligopeptides and DNA Mimics the Sequential Disassembly of a Spherical Virus

Ni Rong; Chau Ying

摘要

Abstract >Protein subunits of a low aspect ratio (width over length) with stimuli‐responsiveness are hallmark building blocks of spherical viruses. The interaction of these repeating subunits enables hierarchical assembly for genome packaging and sequential disassembly for optimal genome release. Here, we mimicked these features and constructed a functional spherical artificial virus. The rationally designed 22‐amino acid peptide containing pH‐sensitive histidines and aromatic residues self‐assembled into homogenous nanodiscs of a low aspect ratio (≈7×7×4?nm). In the presence of DNA, the inter‐nanodisc interactions drove the formation of a viral capsid‐like structure enclosing DNA in the interior. This artificial virus roughly 50?nm in diameter underwent partial disassembly in response to acidic pH. The resulting intermediate with lowered DNA‐binding affinity continued to protect DNA from nuclease digestion. Such nanostructures, which mimic the intricate morphology and the intracellular transformation of spherical viruses, can be useful for constructing synthetic gene delivery vehicles, as evidenced by their efficient transgene expression. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >具有刺激反应性的低纵横比（宽度超过长度）的蛋白质亚基是球形病毒的标志构建块。这些重复亚基的相互作用使得能够用于基因组包装和顺序拆卸以获得最佳基因组释放的分层组装。在这里，我们模仿这些特征并构建了一个功能性球形人工病毒。理性设计的22-氨基酸肽含有pH敏感组氨酸和芳族残基以低纵横比（≈7×7×4×4×4×4×4×4）自组装成均质纳米块。在DNA存在下，纳米氏蛋白酶相互作用驱动了在内部封闭DNA的病毒衣壳状结构的形成。这种人工病毒直径大约50？nm响应于酸性pH的部分拆卸。所得中间体具有降低的DNA结合亲和力继续保护DNA免受核酸酶消化。这种纳米结构模仿复杂形态和球形病毒的细胞内转化，可用于构建合成基因递送载体，如其有效转基因表达所证明的。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-19011/'>《Angewandte Chemie》</a> <b style="margin: 0 2px;">|</b><span>2020年第9期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ni Rong&option=202" target="_blank" rel="nofollow">Ni Rong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chau Ying&option=202" target="_blank" rel="nofollow">Chau Ying;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Chemical and Biological EngineeringThe Hong Kong University of Science and TechnologyClearwater Bay Kowloon Hong Kong China;</p> <p>Department of Chemical and Biological EngineeringThe Hong Kong University of Science and TechnologyClearwater Bay Kowloon Hong Kong China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1188.html" title="应用化学">应用化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=artificial viruses&option=203" rel="nofollow">artificial viruses;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=DNA&option=203" rel="nofollow">DNA;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=gene technology&option=203" rel="nofollow">gene technology;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=peptides&option=203" rel="nofollow">peptides;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=spherical viruses&option=203" rel="nofollow">spherical viruses;</a> </p> <div class="translation"> 机译：人工病毒;DNA;基因技术;肽;球形病毒; 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李锐&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李锐</a> <span> . 2007</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120971938.html">一种从制备纳米球形磷酸铁到碳融合法连续制备纳米球形磷酸铁锂的方法</a> <b>[P]</b> . <span> 中国专利： CN102255078B </span> <span> . 2012.07.18</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120106452203.html">一种从制备纳米球形磷酸铁到碳融合法连续制备纳米球形磷酸铁锂的方法</a> <b>[P]</b> . <span> 中国专利： CN102255078A </span> <span> . 2011-11-23</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130472325368.html">B-TYPE HEPATITIS VIRUS, DNA FRAGMENT, POLYPEPTIDE AND OLIGOPEPTIDE FROM B-TYPE HEPATITIS VIRUS COMPONENT, PRODUCTION OF RECOMBINED DNA MOLECULE, DNA VECTOR, ESHERICIACOLI K12 VARIANT AND POLYPEPTIDE, INNOCULANT SUBSTANCE TO B-TYPE HEPATITIS VIRUS DISEASE, MONOCRONAL ANTIBODY MA18/7 AND MOUSE HYBRID CELL GOLA18/7-1 AND PRODUCTION OF MONOCRONAL ANTIBODY MA 187</a> <b>[P]</b> . <span> 外国专利：  JPS61111695A </span> <span> . 1986-05-29</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：B型肝炎病毒，B型肝炎病毒组分的DNA片段，多肽和寡肽，重组DNA分子，DNA矢量，大肠杆菌K12变异蛋白和多肽的生产，对B型肝炎病毒的无活性/突变，这是一种B型肝炎和小鼠混合细胞GOLA18 / 7-1与单克隆抗体MA 187的生产 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130452645655.html">IDENTIFIED DNA SEQUENCES CODING L1 AND L2 CAPSIDE PROTEINS OF HUMAN PAPYPTHEMA VIRUS TYPE 18, VECTOR FOR EXPRESSING DNA SEQUENCE, A METHOD FOR OBTAINING VIRUS-SUITABLE PARTICLES AND FITTED CURRENT CURRENT CURRENT UNIT CURRENCY CODES, CURRENT USE OF VIRUS-BASED PARTICLES OF DNA VIRTUAL TYPES, A METHOD FOR OBTAINING VIRUS-SUITABLE PARTICLES, TYPES OF DIRECTIONAL TREATMENT, TYPE OF VIRUS-SECOND CURRENT USE OF DNA VIRTUAL CODES</a> <b>[P]</b> . <span> 外国专利：  EA199700256A1 </span> <span> . 1999-06-24</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：识别编码人乳头状瘤病毒18型L1和L2蛋白酶蛋白的DNA序列，用于表达DNA序列的矢量，一种获取病毒合适颗粒的方法，并根据当前流通的当前DNA密码子的正确性，从当前的计算机中提取出正确的数字，获取病毒适合颗粒的方法，方向处理类型，DNA虚拟代码当前使用病毒的类型 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130461990623.html">Process for production of virus DNA citoplasmico eucariotico modified virus DNA citoplasmico eucariotico modidicado, molecule of DNA.Set of components for direct molecular cloning of a genomaviral modified a virus DNA citoplasmico eucariotico, plasmideo and virus vaccine modified</a> <b>[P]</b> . <span> 外国专利：  BR9203322A </span> <span> . 1993-03-30</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：制备DNA修饰的病毒DNA的方法，DNA分子。基因组病毒修饰的直接DNA克隆分子组，修饰的DNA修饰的质粒，质粒和病毒疫苗 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028160970','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div 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