Rational Design of a Two‐Photon Fluorogenic Probe for Visualizing Monoamine Oxidase?A Activity in Human Glioma Tissues

Fang Haixiao; Zhang Hang; Li Lin; Ni Yun; Shi Riri; Li Zheng; Yang Xuekang; Ma Bo; Zhang Chengwu; Wu Qiong; Yu Changmin; Yang Naidi; Yao Shao Q.; Huang Wei

摘要

Abstract > Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO‐A and MAO‐B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO‐A‐specific two‐photon fluorogenic probe ( F1 ), capable of selective imaging of endogenous MAO‐A enzymatic activities from a variety of biological samples, including MAO‐A‐expressing neuronal SY‐SY5Y cells, the brain of tumor‐bearing mice and human Glioma tissues by using two‐photon fluorescence microscopy (TPFM) with minimal cytotoxicity. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 单胺氧化酶具有两种功能性明显但结构性相似的同种型（Mao-A和MaO-B）。通过使用荧光检测/成像技术将它们区分的能力具有显着的生物相关性，但具有高度挑战，可用的化学工具。在此，我们报告了第一个MAO-A特异性双光子荧光探针（ f1 </ b> ），能够通过使用双光子荧光显微镜（包括Mao-A表达的神经元SY5Y细胞，包括MAO-A-A-表达神经元SY5Y细胞的酶活性的内源性MAO-A酶活性的成像。通过使用双光子荧光显微镜（TPFM）具有最小的细胞毒性。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-19011/'>《Angewandte Chemie》</a> <b style="margin: 0 2px;">|</b><span>2020年第19期</span><b style="margin: 0 2px;">|</b><span>共6页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fang Haixiao&option=202" target="_blank" rel="nofollow">Fang Haixiao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Hang&option=202" target="_blank" rel="nofollow">Zhang Hang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Lin&option=202" target="_blank" rel="nofollow">Li Lin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ni Yun&option=202" target="_blank" rel="nofollow">Ni Yun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shi Riri&option=202" target="_blank" rel="nofollow">Shi Riri;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Zheng&option=202" target="_blank" rel="nofollow">Li Zheng;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Xuekang&option=202" target="_blank" rel="nofollow">Yang Xuekang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ma Bo&option=202" target="_blank" rel="nofollow">Ma Bo;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Chengwu&option=202" target="_blank" rel="nofollow">Zhang Chengwu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wu Qiong&option=202" target="_blank" rel="nofollow">Wu Qiong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yu Changmin&option=202" target="_blank" rel="nofollow">Yu Changmin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Naidi&option=202" target="_blank" rel="nofollow">Yang Naidi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yao Shao Q.&option=202" target="_blank" rel="nofollow">Yao Shao Q.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huang Wei&option=202" target="_blank" rel="nofollow">Huang Wei;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Key Laboratory of Flexible Electronics (KLOFE) &</p> <p>Institute of Advanced Materials (IAM)Nanjing Tech University (NanjingTech)30 South Puzhu Road Nanjing 211816 P. R. China;</p> <p>Key Laboratory of Flexible Electronics (KLOFE) &</p> <p>Institute of Advanced Materials (IAM)Nanjing Tech University (NanjingTech)30 South Puzhu Road Nanjing 211816 P. R. China;</p> <p>Key Laboratory of Flexible Electronics (KLOFE) &</p> <p>Institute of Advanced Materials (IAM)Nanjing Tech University (NanjingTech)30 South Puzhu Road Nanjing 211816 P. R. China;</p> <p>Key Laboratory of Flexible Electronics (KLOFE) &</p> <p>Institute of Advanced Materials (IAM)Nanjing Tech University (NanjingTech)30 South Puzhu Road Nanjing 211816 P. R. China;</p> <p>Key Laboratory of Flexible Electronics (KLOFE) &</p> <p>Institute of Advanced Materials (IAM)Nanjing Tech University (NanjingTech)30 South Puzhu Road Nanjing 211816 P. R. China;</p> <p>Key Laboratory of Flexible Electronics (KLOFE) &</p> <p>Institute of Advanced Materials (IAM)Nanjing Tech University (NanjingTech)30 South Puzhu Road Nanjing 211816 P. R. China;</p> <p>Department of Burns and Cutaneous SurgeryThe Fourth Military Medical UniversityXi'an 710032 P. R. China;</p> <p>School of Pharmaceutical SciencesNanjing Tech UniversityNanjing 210023 P. R. China;</p> <p>Key Laboratory of Flexible Electronics (KLOFE) &</p> <p>Institute of Advanced Materials (IAM)Nanjing Tech University (NanjingTech)30 South Puzhu Road Nanjing 211816 P. R. China;</p> <p>Key Laboratory of Flexible Electronics (KLOFE) &</p> <p>Institute of Advanced Materials (IAM)Nanjing Tech University (NanjingTech)30 South Puzhu Road Nanjing 211816 P. R. China;</p> <p>Key Laboratory of Flexible Electronics (KLOFE) &</p> <p>Institute of Advanced Materials (IAM)Nanjing Tech University (NanjingTech)30 South Puzhu Road Nanjing 211816 P. R. China;</p> <p>Key Laboratory of Flexible Electronics (KLOFE) &</p> <p>Institute of Advanced Materials (IAM)Nanjing Tech University (NanjingTech)30 South Puzhu Road Nanjing 211816 P. R. China;</p> <p>Department of ChemistryNational University of Singapore3 Science Drive 3 117543 Singapore Singapore;</p> <p>Key Laboratory of Flexible Electronics (KLOFE) &</p> <p>Institute of Advanced Materials (IAM)Nanjing Tech University (NanjingTech)30 South Puzhu Road Nanjing 211816 P. R. China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1188.html" title="应用化学">应用化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bioimaging&option=203" rel="nofollow">bioimaging;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fluorogenic probes&option=203" rel="nofollow">fluorogenic probes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=glioma&option=203" rel="nofollow">glioma;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=monoamine oxidase?A&option=203" rel="nofollow">monoamine oxidase?A;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=two-photon fluorescence microscopy&option=203" rel="nofollow">two-photon fluorescence microscopy;</a> </p> <div class="translation"> 机译：BioMimaging;荧光探针;胶质瘤;单胺氧化酶？a;双光子荧光显微镜; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704028160853.html">Rational Design of a Two‐Photon Fluorogenic Probe for Visualizing Monoamine 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href="/conference-cn-43299/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第十一届中国酶工程学术研讨会 </a> <span> <span> . 2017</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020312571221.html">硫化氢及相关酶活性的双光子荧光探针设计与成像分析</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈倩&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 陈倩</a> <span> . 2016</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061204467669.html">一种双光子比率型荧光探针在检测单胺氧化酶B中的应用</a> <b>[P]</b> . <span> 中国专利： CN110218215B </span> <span> . 2021.06.15</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120101075452.html">一种双光子比率型荧光探针在检测单胺氧化酶B中的应用</a> <b>[P]</b> . <span> 中国专利： CN110218215A </span> <span> . 2019-09-10</span> </div> </li> <li> <div> <b>3. </b><a 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