Asymmetric β‐Methylation of l‐ and d‐α‐Amino Acids by a Self‐Contained Enzyme Cascade

Liao Cangsong; Seebeck Florian P.

摘要

Abstract > This report describes a modular enzyme‐catalyzed cascade reaction that transforms <sc>l</sc> ‐ or <sc>d</sc> ‐α‐amino acids to β‐methyl‐α‐amino acids. In this process an α‐amino acid transaminase, an α‐keto acid methyltransferase, and a halide methyltransferase cooperate in two orthogonal reaction cycles that mediate product formation and regeneration of the cofactor pyridoxal‐5′‐phosphate and the co‐substrate S‐adenosylmethionine. The only stoichiometric reagents consumed in this process are the unprotected <sc>l</sc> ‐ or <sc>d</sc> ‐α‐amino acid and methyl iodide. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 本报告描述了转化的模块化酶催化级联反应 <sc> l </ sc> - 或者 <sc> d </ sc> -α-氨基酸至β-甲基-α-氨基酸。在该方法中，α-氨基酸转氨酶，α-酮酸甲基转移酶和卤化物甲基转移酶在两个正交反应循环中配合，其介导产品的形成和再生乙酰吡哆醛-5'-磷酸盐和共底族腺苷甲硫氨酸。在这个过程中消耗的唯一化学计量试剂是未受保护的 <sc> l </ sc> - 或者 <sc> d </ sc> -α-氨基酸和甲基碘化物。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-19011/'>《Angewandte Chemie》</a> <b style="margin: 0 2px;">|</b><span>2020年第18期</span><b style="margin: 0 2px;">|</b><span>共4页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liao Cangsong&option=202" target="_blank" rel="nofollow">Liao Cangsong;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Seebeck Florian P.&option=202" target="_blank" rel="nofollow">Seebeck Florian P.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department for ChemistryUniversity of BaselMattenstrasse 24a BPR 1002 4056 Basel Switzerland;</p> <p>Department for ChemistryUniversity of BaselMattenstrasse 24a BPR 1002 4056 Basel Switzerland;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1188.html" title="应用化学">应用化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=amino acids&option=203" rel="nofollow">amino acids;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=biocatalysis&option=203" rel="nofollow">biocatalysis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=methyltransferase&option=203" rel="nofollow">methyltransferase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=S-adenosylmethionine&option=203" rel="nofollow">S-adenosylmethionine;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=stereoselective C-alkylation&option=203" rel="nofollow">stereoselective C-alkylation;</a> </p> <div class="translation"> 机译：氨基酸;生物催化;甲基转移酶;S-腺苷甲硫氨酸;立体选择性C-烷基化; 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style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201295723946.html">D—氨基酸在人体的来龙去脉──人体中D－氨基酸、D－氨基酸氧化酶、D－天冬氨酸氧化酶研究进展</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=赵南生&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 赵南生</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=余志立&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,余志立</a> <span> <a href="/journal-cn-57107/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 氨基酸和生物资源 </a> </span> <span> . 1995</span><span>,第2期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201295687925.html">重组大肠杆菌共表达D-海因酶和N-氨甲酰基-D-氨基酸酰胺水解酶</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=胡晓佳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 胡晓佳</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李强&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李强</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=丛进阳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,丛进阳</a> <span> <a href="/journal-cn-56742/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 过程工程学报 </a> </span> <span> . 2006</span><span>,第6期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201295706654.html">高活力固定化酰化氨基酸水解酶的制备及其在L-氨基酸和D-氨基酸生产中的应用</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=韩沾元&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 韩沾元</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=谭佩幸&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,谭佩幸</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄新弟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,黄新弟</a> <span> <a href="/journal-cn-57029/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 化学试剂 </a> </span> <span> . 1992</span><span>,第1期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-applied-chemistry_thesis/0201277648260.html">D-扁桃酸脱氢酶和L-亮氨酸脱氢酶级联催化的L-苯甘氨酸对映选择性生物合成</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=史红玲&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 史红玲</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王文杰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王文杰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李祥&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李祥</a> <span> <a href="/journal-cn-54377/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 应用化学 </a> </span> <span> . 2020</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201295605613.html">D-氨基酸氧化酶/D-丙氨酸系统杀伤K562e细胞的作用机制探讨</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=翟勇平&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 翟勇平</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王健民&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王健民</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张雨生&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张雨生</a> <span> <a href="/journal-cn-56698/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第二军医大学学报 </a> </span> <span> . 2002</span><span>,第2期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-29161_thesis/020222146821.html">D-氨基酸氧化酶和戊二酰-7-氨基头孢烷酸酰化酶的表达纯化和固定化</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=郑华宝&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 郑华宝</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王筱兰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王筱兰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈军&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈军</a> <span> <a href="/conference-cn-29161/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第四届中国酶工程学术交流讨论会 </a> <span> <span> . 2003</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020313790862.html">工程菌生物转化D-型氨基酸的研究——D-海因酶基因的克隆、表达、纯化及其性质</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=封霞&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 封霞</a> <span> . 2004</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120104654824.html">新型DNA编码的D－酰化氨基酸水解酶以及将其用于生产D－氨基酸的方法</a> <b>[P]</b> . <span> 中国专利： CN1457362A </span> <span> . 2003-11-19</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120104766077.html">用D－氨基酸氧化酶和D－天冬氨酸氧化酶拮抗剂治疗中枢神经系统障碍</a> <b>[P]</b> . <span> 中国专利： CN1568370A </span> <span> . 2005-01-19</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130449108062.html">Nucleic acid fragment isolated chimeric gene, host cell transformed polipeptu00ecdeo of dihidroxiu00e1cido desidratase, polipeptu00ecdeo branched chain amino acid aminotransferase.Leuc subunit and subunit leud of polipeptu00ecdeo 3 - isopropilmalato desidratase,Method for modification of the level of expression of branched chain amino acid biosynthetic enzyme in a host cell, method of obtaining nucleic acid fragment and its product.And assessment method of the compound for its ability to inhibit the activity of a biosynthetic enzyme of branched chain amino acids</a> <b>[P]</b> . <span> 外国专利：  BR9815228A </span> <span> . 2001-01-02</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：核酸片段分离的嵌合基因，宿主细胞转化的双歧双歧杆菌脱氢酶，多聚支链氨基酸转氨酶。多聚核苷酸的Leuc亚基和亚基3-异丙基丙二酸脱氢酶，表达水平的修饰方法宿主细胞中支链氨基酸生物合成酶的制备，核酸片段及其产物的制备方法以及该化合物抑制支链氨基酸生物合成酶活性的能力的评价方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130417748684.html">Processes for the preparation of D - 1,2,4 - butanotriol trinitrate 1,2,4 - butanotriol, acid (D - 3.4 - diidroxibutanu00d3ico, acid 3 - desoxi - D - gliceropentanu00d3ico, acid (4S) - 2 - amino - 4 - diidroxipentanu00d3ico; D - 1,2,4 - butanotriol trimethylolethane (D - 1,2,4 - butanotriol, D - xylose dehydrogenase, acid. D - xilu00d4nico, desidratase acid, 3 - desoxi - gliceropentanu00d3ico acid, D - 3 - 4 - diidroxibutanu00d3ico,Acid (4S) - 2 - amino - 4 - diidroxipentanu00d3ico; acids nuclu00c9icos encoders of a D - xylose or recombinant for biossu00cdntese of 1,2,4 - butanotriol, caffeic acid (4S) - 2 - amino - 4 - diidroxipentanu00d3ico, acid (D - 3 - 4 - diidroxibutanu00d3ico, caffeic acid (4S) - 2 - amino - 4.5 - diidroxipentanu00d3ico vector knock-out; A 3 - desoxi - D - glicero - pentulossonato aldolase, entity cell recombinant, cu00c9lula recombinant.The process for research of polinucleotu00cddeos candidates encoding enzymes, antibody.</a> <b>[P]</b> . <span> 外国专利：  BRPI0714439A2 </span> <span> . 2014-04-08</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：D-1,2,4-丁酸三硝酸丁酯1,2,4-丁酸的制备方法（D-3.4-diidroxibutan u00d3ico，酸3-脱氧-D-gliceropentan u00d3ico，酸（4S）-2 -氨基-4-diidroxipentan u00d3ico; D-1,2,4-丁三醇三羟甲基乙烷（D-1,2,4-丁三醇，D-木糖脱氢酶，酸.D-xil u d00nico，desidratase酸，3-脱氧- gliceropentan u00d3ico酸，D-3-4-二溴代丁烷 u00d3ico，酸（4S）-2-氨基-4-diidroxipentan u00d3ico; D-木糖或重组体的酸nucl u00c9icos编码器，1,2 ，4-丁三醇，咖啡酸（4S）-2-氨基-4-二溴代西an丹 u00d3ico，酸（D-3-4-diidroxibutan u00d3ico，咖啡酸（4S）-2-氨基-4.5-diidroxipentan u00d3ico -out; A 3-去氧-D -glicero-戊糖醛酸钠醛缩酶，实体细胞重组体，c9重组体。研究编码酶，抗体的多核苷酸候选物的方法。 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130425481490.html">The composition of nucleic acid enzymes multi-component (mnazima), methods for detecting the presence of at least one facilitator for combination, a target, a variant of the sequence of nucleic acids, for detecting the presence of at least one methylated nucleic acid and to detect sp Least a facilitator of combination using a cascade of expansion.Methods for fabricating a plurality of nucleic acid enzymes multi-component (mnazima), and the use of at least one oligonucleotu00ecdeo</a> <b>[P]</b> . <span> 外国专利：  BRPI0616466A2 </span> <span> . 2011-06-21</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：核酸酶多组分的组合物（疟疾），检测至少一种组合促进剂的存在的方法，靶标，核酸序列的变体，用于检测至少一种甲基化核酸的存在和通过级联扩增来检测sp，从而至少是一种促进组合物的方法。多种核酸酶多组分（mnazima）的制备方法，以及至少一种寡核苷酸的使用 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028160795','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div 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