Untargeted Tumor Metabolomics with Liquid Chromatography–Surface‐Enhanced Raman Spectroscopy

Xiao Lifu; Wang Chuanqi; Dai Chen; Littlepage Laurie E.; Li Jun; Schultz Zachary D.

摘要

Abstract >Metabolomics is a powerful systems biology approach that monitors changes in biomolecule concentrations to diagnose and monitor health and disease. However, leading metabolomics technologies, such as NMR and mass spectrometry (MS), access only a small portion of the metabolome. Now an approach is presented that uses the high sensitivity and chemical specificity of surface‐enhanced Raman scattering (SERS) for online detection of metabolites from tumor lysates following liquid chromatography (LC). The results demonstrate that this LC‐SERS approach has metabolite detection capabilities comparable to the state‐of‐art LC‐MS but suggest a selectivity for the detection of a different subset of metabolites. Analysis of replicate LC‐SERS experiments exhibit reproducible metabolite patterns that can be converted into barcodes, which can differentiate different tumor models. Our work demonstrates the potential of LC‐SERS technology for metabolomics‐based diagnosis and treatment of cancer. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >代谢组学是一种强大的系统生物学方法，可以监测生物分子浓度的变化，以诊断和监测健康和疾病。然而，领先的代谢组科技术，例如NMR和质谱（MS），仅进入代谢物的一小部分。现在提出了一种方法，其利用表面增强拉曼散射（SERs）的高灵敏度和化学特异性来在液相色谱（LC）之后从肿瘤裂解物的在线检测代谢物。结果表明，该LC-SERS方法具有与最先进的LC-MS相当的代谢物检测能力，但表明了检测不同代谢物子集的选择性。复制LC-SERS实验的分析表现出可转化为条形码的可重复的代谢物模式，其可以区分不同的肿瘤模型。我们的作品展示了基于代谢组织的诊断和治疗癌症的LC-SERS技术的潜力。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-19011/'>《Angewandte Chemie》</a> <b style="margin: 0 2px;">|</b><span>2020年第9期</span><b style="margin: 0 2px;">|</b><span>共5页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xiao Lifu&option=202" target="_blank" rel="nofollow">Xiao Lifu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Chuanqi&option=202" target="_blank" rel="nofollow">Wang Chuanqi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dai Chen&option=202" target="_blank" rel="nofollow">Dai Chen;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Littlepage Laurie E.&option=202" target="_blank" rel="nofollow">Littlepage Laurie E.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Jun&option=202" target="_blank" rel="nofollow">Li Jun;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Schultz Zachary D.&option=202" target="_blank" rel="nofollow">Schultz Zachary D.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Chemistry and BiochemistryThe Ohio State UniversityColumbus OH 43210 USA;</p> <p>Department of Applied and Computational Mathematics and StatisticsUniversity of Notre DameNotre Dame IN 46556 USA;</p> <p>Department of Chemistry and BiochemistryUniversity of Notre DameNotre Dame IN 46556 USA;</p> <p>Department of Chemistry and BiochemistryUniversity of Notre DameNotre Dame IN 46556 USA;</p> <p>Department of Applied and Computational Mathematics and StatisticsUniversity of Notre DameNotre Dame IN 46556 USA;</p> <p>Department of Chemistry and BiochemistryThe Ohio State UniversityColumbus OH 43210 USA;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1188.html" title="应用化学">应用化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=LC-SERS&option=203" rel="nofollow">LC-SERS;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=metabolic fingerprinting&option=203" rel="nofollow">metabolic fingerprinting;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=metabolomics&option=203" rel="nofollow">metabolomics;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=surface-enhanced Raman scattering&option=203" rel="nofollow">surface-enhanced Raman scattering;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=tumors&option=203" rel="nofollow">tumors;</a> </p> <div class="translation"> 机译：lc-sers;代谢指纹识别;代谢组合;表面增强拉曼散射;肿瘤; 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R. </a> <span>2015</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：拉曼光谱与多组分搜索情结秘密实验室样品分析（一），拉曼光谱为（II）甲基苯丙胺的秘密实验室液体的快速，无损筛选试验和拉曼光谱鉴定为增强合成卡西分析（三）。</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_electronic-journal-metabolism-nutrition-cancer_thesis/0201261821339.html">《肿瘤代谢与营养电子杂志》被中国学术期刊网络出版总库全文收录《肿瘤代谢与营养电子杂志》被中国核心期刊(遴选)数据库收录《肿瘤代谢与营养电子杂志》被中文科技期刊数据库全文收录</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/journal-cn-38755/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 肿瘤代谢与营养电子杂志 </a> </span> <span> . 2015</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_electronic-journal-metabolism-nutrition-cancer_thesis/0201261824304.html">《肿瘤代谢与营养电子杂志》被中国学术期刊网络出版总库全文收录《肿瘤代谢与营养电子杂志》被中国核心期刊(遴选)数据库收录《肿瘤代谢与营养电子杂志》被中文科技期刊数据库全文收录</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/journal-cn-38755/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 肿瘤代谢与营养电子杂志 </a> </span> <span> . 2015</span><span>,第004期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-journal-analytical-chemistry_thesis/0201230629247.html">茅莓黄酮液相色谱-电喷雾质谱与薄层色谱-表面增强拉曼光谱研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=汪瑗&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 汪瑗</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=贺玖明&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,贺玖明</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈惠&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈惠</a> <span> <a href="/journal-cn-10804/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 分析化学 </a> </span> <span> . 2006</span><span>,第008期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-chemical-physics_thesis/0201293166691.html">用于低丰度添加剂检测的具有表面增强拉曼光谱活性的Au@Au@Ag双壳核纳米粒子的制备</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=魏文娴&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 魏文娴</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=奚钟钱&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,奚钟钱</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄庆利&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,黄庆利</a> <span> <a href="/journal-cn-12046/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 化学物理学报 </a> </span> <span> . 2021</span><span>,第2期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-liaoning-normal-university-natural-science-edition_thesis/0201250096930.html">具有表面增强拉曼光谱光纤探针的研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨修文&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杨修文</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=祝生祥&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,祝生祥</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王绍明&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王绍明</a> <span> <a href="/journal-cn-4664/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 辽宁师范大学学报（自然科学版） </a> </span> <span> . 2007</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-8075_thesis/020222071623.html">具有触头测温功能的电子式互感器与隔离开关组合敞开式智能组合开关研发和工程运用</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fan Rongquan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 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