Synthesis of dTBP-6-Deoxy-4-ketoghscose and Analogues with Native and Rrcombinant dTDP-Glucose-4,6-dehydratase

摘要

Activated ketoglucose derivatives play a key role in the biosynthesis of deoxygenated oligo- and polysaccharides, because they represent the branching points in many biosynthetie pathways leading to 2,6- and 3,6-dideoxy- and -amincdeoxysug-ars. and branched deoxysugars Although the gene clusters for the biosynthesis of deoxysugars in bacteria have been localized and the corresponding enzymes should now be available, the insufficient availability of the intermediates arid the lack of effective analytical methods still hampers detailed investigations. Solving these problems is necessary before the chemoenzymatic synthesis of deoxyoligosaccharides is as successful as that of N-glycosidicaily bound giycans.