Characterization of the photosynthetic system (Ultrastructure of plastids,fluorescence induction profiles, low temperature spectra) of Arachishypogaea L-callus cultures as influenced by sucrose and various hormonaltreatments

摘要

Freshly cut hypocotyl explants and established Arachis hypogaea L. (peanut) callus cultures behaved differently to the hormonal supplement of the nutritional medium. A hormonal response was more pronounced in case of freshly isolated explants than in long established cultures apparently because the later developed their own hormonal system and thereby responded poorly to exogenous phytohormones The photosynthetic system of established cultures which proved to be photoautotrophic was characterized in the present paper by ultrastructural investigations, low temperature absorption spectra and fluoressence induction measurements at various hormonal regimes. Whereas influences of exogenous phytohormones were rather small, a supplement of sucrose to the nutrient medium has suppressive effects on the development of the photosynthetic system.