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Generation of Equally Sized Particle Plaques Using Solid-Liquid Suspensions

机译:使用固液悬浮液产生均等大小的颗粒斑块

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摘要

A device is presented for the generation of equally sized plaques of sensitive particles in a 96-well format.The resulting particle plaques can be used for the measurement of adsorption isotherms and uptake kinetics in protein chromatography or for immobilization reactions.The particle plaques are formed from suspensions with a vacuum device that is designed as a reusable sandwich module.The particles are retained by a mesh while the solvent is removed by the vacuum.As most particles used for protein chromatography are sensitive to mechanical stress and dehydration,the vacuum device is gentle enough to allow the use of these particles,thus eliminating the uncertainty of slurry preparation and pipetting.Apparatus characteristics and preparation procedures are described precisely.The physical intactness of the particles after the preparation procedure is proved by microscopic analysis.Data on the uniformity of the obtained;resin plaques with respect to the reproducibility of their adsorption performance is given.Finally,adsorption isothermal and kinetic data of BSA on an ordinary HIC system obtained by high-throughput measurements are shown as an application example.
机译:提出了一种用于以96孔格式生成大小相等的敏感颗粒斑块的设备,该颗粒斑块可用于测量蛋白质色谱中的吸附等温线和吸收动力学或用于固定化反应。由带有可重复使用的三明治模块的真空装置的悬浮液制成。颗粒被筛网保留,而溶剂被真空除去。由于大多数用于蛋白质色谱的颗粒对机械应力和脱水敏感,因此真空装置是足够温和以允许使用这些颗粒,从而消除了制浆和移液的不确定性。精确描述了设备的特性和制备步骤。制备过程后颗粒的物理完整性通过显微镜分析得到了证明。所获得的树脂斑块关于其吸附剂的可复制性最后给出了通过高通量测量获得的普通HIC系统上BSA的吸附等温和动力学数据作为应用实例。

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