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Sample preparation strategies for high‐throughput mass spectrometry imaging of primary tumor organoids

机译：用于原发性肿瘤有机体高通量质谱成像的样品制备策略

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Abstract >Patient‐derived 3D organoids show great promise for understanding patient heterogeneity and chemotherapy response in human‐derived tissue. The combination of organoid culture techniques with mass spectrometry imaging provides a label‐free methodology for characterizing drug penetration, patient‐specific response, and drug biotransformation. However, current methods used to grow tumor organoids employ extracellular matrices that can produce small molecule background signal during mass spectrometry imaging analysis. Here, we develop a method to isolate 3D human tumor organoids out of a Matrigel extracellular matrix into gelatin mass spectrometry compatible microarrays for high‐throughput mass spectrometry imaging analysis. The alignment of multiple organoids in the same z‐axis is essential for sectioning organoids together and for maintaining reproducible sample preparation on a single glass slide for up to hundreds of organoids. This method successfully removes organoids from extracellular matrix interference and provides an organized array for high‐throughput imaging analysis to easily identify organoids by eye for area selection and further analysis. With this method, mass spectrometry imaging can be readily applied to organoid systems for preclinical drug development and personalized medicine research initiatives. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >患者衍生的3D细胞器对理解人源性组织中的患者的异质性和化疗反应表现出良好的希望。有机体培养技术与质谱成像的组合提供了无标记的方法，用于表征药物渗透，患者特异性反应和药物生物转化。然而，用于生长肿瘤有机体的当前方法采用细胞外基质，其在质谱期间可以产生小分子背景信号。在这里，我们开发一种方法，将3D人肿瘤有机体从Matrigel细胞外基质中分离出明胶质谱相容的微阵列进行高通量质谱成像分析。在相同Z轴上的多个有机体的对准对于切片有机体在一起并将可再现的样品制剂保持在单个玻璃载玻片上，以便高达数百个有机体。该方法成功地从细胞外基质干扰中去除有机体，并为高通量成像分析提供有机化的阵列，以便通过眼睛识别有机体以进行区域选择和进一步的分析。利用这种方法，可以容易地应用质谱成像以用于临床前药物开发和个性化医学研究举措的有机体系。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-34906/'>《Journal of mass spectrometry: JMS》</a> <b style="margin: 0 2px;">|</b><span>2020年第4期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Johnson Jillian&option=202" target="_blank" rel="nofollow">Johnson Jillian;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sharick Joe T.&option=202" target="_blank" rel="nofollow">Sharick Joe T.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Skala Melissa C.&option=202" target="_blank" rel="nofollow">Skala Melissa C.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Li Lingjun&option=202" target="_blank" rel="nofollow">Li Lingjun;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>School of PharmacyUniversity of Wisconsin—MadisonMadison Wisconsin;</p> <p>Morgridge Institute for ResearchMadison Wisconsin;</p> <p>Morgridge Institute for ResearchMadison Wisconsin;</p> <p>School of PharmacyUniversity of Wisconsin—MadisonMadison Wisconsin;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/1187.html" title="分析化学">分析化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=drug discovery&option=203" rel="nofollow">drug discovery;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=high throughput&option=203" rel="nofollow">high throughput;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=MALDI MSI&option=203" rel="nofollow">MALDI MSI;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=microwells&option=203" rel="nofollow">microwells;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=organoids&option=203" rel="nofollow">organoids;</a> </p> <div class="translation"> 机译：药物发现;高吞吐量;马尔迪MSI;微孔;有机体; 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