Evaluation of Diazinon Toxicity on Nile Tilapia Fish (O. niloticus)

摘要

Diazinon was used in the laboratorial study to investigate its biochemical effect on tilapia as it is the most popular fish in Egypt. Two hundred and twenty appeared 40 plus or minus 2 g adult male Nile tilapia were reared in glass aquaria of 60 L capacity, provided with a good air supply and dechlorinated tap water, Fish were maintained under suitable condition for the fish growth. Results of the study are summarized as follow: (1) The bioassay test revealed that the LC sub(50) for tilapia after 96 h of exposure was 2.8 ppm, (2) Fish was very excited after being exposed to lethal concentrations of diazinon (5, 10, 200 ppm) for 96 h. Meanwhile, fish exposed to sublethal concentrations of diazinon for 30 days didn't cause mortality to fish and (3) Exposing fish to 0.28 and 1.87 ppm for 30 days caused the following changes: (A) A reduction in total protein content in muscles up to 13.69 and 21.5% for 0.28 and 1.87 ppm, respectively, (B) A reduction in total protein content in blood serum up to 22.23 and 24.32% for 0.28 and 1.87 ppm, respectively and(c) 52, 27 and 6.8 kDa proteins were not scanned in the treated or the recovered samples in both treatments, a slight reduction in the 33.55, 31.72, 24.31 and 20.8 kDa proteins in both treatments, (4) Exposing the treated fish to 7 days of recovery in un poisoned water caused the following changes: (A) A recovery in total protein content in muscles up to 95.59 and 90.58% for 0.28 and 1.87 ppm, respectively, (B) A recovery in total protein content in blood serum up to 89.36 and 95.14% for 0.28 and 1.87 ppm, respectively and (C) 52.27 and 6.8 kDa proteins were still not scanned after recovery of both treatments. A slight increase in the rest of affected proteins after recovery of both treatments was recorded. Therefore, it can be emphasized for good environmental administration of the water bodies to save human health and environment from the dangerous pesticides.