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Detoxification of dilute acid hydrolysates of lignocellulose with lime

机译:用石灰对木质纤维素的稀酸水解产物进行解毒

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摘要

The hydrolysis of hemicellulose to monomeric sugars by dilute acid hydrolysis is accompanied by the production of inhibitors that retard microbial fermentation. Treatment of hot hydrolysate with Ca(OH)(2) (overliming) is an effective method for detoxification. Using ethanologenic Escherichia coli LY01 as the biocatalyst, our results indicate that the optimal lime addition for detoxification varies and depends on the concentration of mineral acids and organic acids in each hydrolysate. This optimum was shown to be readily predicted on the basis of the titration of hydrolysate with 2 N NaOH at ambient temperature to either pH 7.0 or pH 11.0. The average composition of 15 hydrolysates prior to treatment was as follows (per L): 95.24 +/- 7.29 g sugar, 5.3 +/- 2.99 g acetic acid, 1.305 +/- 0.288 g total furans (furfural and hydroxymethylfurfural) and 2.86 +/- 0.34 g phenolic compounds. Optimal overliming resulted in a 51 +/- 9% reduction of total furans, a 41 +/- 6% reduction in phenolic compounds, and a 8.7 +/- 4.5% decline in sugar. Acetic acid levels were unchanged. Considering the similarity of microorganisms, it is possible that the titration method described here may also prove useful for detoxification and fermentation processes using other microbial. biocatalysts.
机译:通过稀酸水解将半纤维素水解为单体糖的过程伴随着阻滞微生物发酵的抑制剂的产生。用Ca(OH)(2)处理热水解产物(过量)是一种有效的排毒方法。使用产乙醇的大肠杆菌LY01作为生物催化剂,我们的结果表明,用于解毒的最佳石灰添加量有所不同,并且取决于每种水解产物中无机酸和有机酸的浓度。根据在环境温度下用2 N NaOH滴定水解产物至pH 7.0或pH 11.0的结果,可以很容易地预测出这一最佳值。处理前15种水解产物的平均组成如下(每升):95.24 +/- 7.29 g糖,5.3 +/- 2.99 g乙酸,1.305 +/- 0.288 g总呋喃(糠醛和羟甲基糠醛)和2.86 + -0.34克酚化合物。最佳的过石灰化处理使总呋喃减少51 +/- 9%,酚类化合物减少41 +/- 6%,糖减少8.7 +/- 4.5%。乙酸水平未改变。考虑到微生物的相似性,此处描述的滴定方法也可能被证明对使用其他微生物的解毒和发酵过程有用。生物催化剂。

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