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Bifunctional adsorbents for hydrophobic displacement chromatography ofproteins

机译:用于蛋白质疏水置换色谱的双功能吸附剂

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摘要

The separation of pancreatic trypsinogen and alpha -chymochypsinogen A by displacement chromatography was tested on a bifunctional adsorbent containing both butyl and carboxymethyl groups. Methacrylic triblock copolymer was synthesized and used as the displacer. Compared to the displacement results on commercial Butyl-Sepharose, it was found that both the separation and recovery of trypsinogen and alpha -chymochypsinogen A were improved. Adsorption isotherms of proteins were measured on both the commercial Butyl-Sepharose and the synthesized bifunctional adsorbents. It was found that the improvement of protein separation on bifunctional adsorbents was attributed to the alteration of the adsorption of trypsinogen. Charge repulsion between trypsinogen and the negatively charged carboxymethyl groups may account for the alteration. In addition, taking advantage of the effect of charge repulsion, the column regeneration became much easier on the column packed with bifunctional adsorbents.
机译:在含有丁基和羧甲基基团的双功能吸附剂上测试了通过置换色谱法分离的胰胰蛋白酶原和α-胰凝乳蛋白酶原A。合成了甲基丙烯酸三嵌段共聚物并用作置换剂。与商业化丁基-琼脂糖的置换结果相比,发现胰蛋白酶原和α-胰凝乳蛋白酶原A的分离和回收率均得到改善。在商用丁基琼脂糖和合成的双功能吸附剂上都测量了蛋白质的吸附等温线。发现双功能吸附剂上蛋白质分离的改善归因于胰蛋白酶原吸附的改变。胰蛋白酶原和带负电荷的羧甲基之间的电荷排斥可能是造成这种变化的原因。此外,利用电荷排斥作用,在装有双功能吸附剂的色谱柱上,色谱柱的再生变得更加容易。

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