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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Corrigendum to “Construction of a highly-active, liver-specific transcriptional regulatory element through combination of the albumin promoter and a-fetoprotein enhancer” [Plasmid 65 (2011) 125-31]
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Corrigendum to “Construction of a highly-active, liver-specific transcriptional regulatory element through combination of the albumin promoter and a-fetoprotein enhancer” [Plasmid 65 (2011) 125-31]

机译:通过白蛋白启动子和A-胎蛋白增强剂组合“通过组合构建高活性,肝细胞特异性转录调节元件”[质粒65(2011)125-31]

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摘要

Construction of a highly-active, liver-specific transcriptional regulatory element through combination of the albumin promoter and a-fetoprotein enhancer.Plasmid, Volume 65, Issue 2, Mar. 2011, Pages 125-31. In this paper, the amplification template of the mouse AFPe and ALBp fragments and the primers for the amplification of the AFPe fragment were described incorrectly. The DNA (not RNA) from the liver tissue of a mouse was used as a template for amplifying the AFPe- and ALBp-containing fragments by PCR (not RT-PCR). And the correct primer sequences for the amplification of the AFPe are as follow: the upstream primer: 5'TGTCCATTCATT AATCCGGAGGCGACTGGGTnGAATnTGC-3'.
机译:通过白蛋白启动子和胎儿蛋白增强剂的组合构建高活性肝细胞特异性转录调节因子。拟合,第65卷,第2月2日,第125-31页。 在本文中,错误地描述了小鼠AFPE和ALBP片段的扩增模板和用于扩增AFPE片段的引物。 来自小鼠的肝脏组织的DNA(不是RNA)用作通过PCR(不是RT-PCR)扩增含AFPE和抗白血病片段的模板。 和扩增AFPE的正确底漆序列如下:上游底漆:5'tgtcTcattcatt AatccggGagcGcGactGGGTNATNTGC-3'。

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