Optimization of the Technology for the Preparation of Cationic Liposomes for the Delivery of Nucleic Acids

摘要

Cationic liposomes based on the cationic lipid 1,26-bis(cholest-5-en-3-yloxycarbonylamino)- 7,11,16-2-tetraazahexacosane tetrahydrochloride (2X3) and the helper lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) have been prepared by two methods, namely ultrasonication and the extrusion of a lipid dispersion. The effect of the preparation method and the type of the water phase on the physicochemical characteristics of cationic liposomes and their biological activity has been studied. The optimization of the preparation of cationic liposomes made it possible to determine the technological parameters providing the reproducibility of physicochemical characteristics of liposomes from batch to batch.