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The Ca2+-Regulated Photoprotein Obelin as a Target for the RNA Aptamer Selection

机译:Ca2 + -regulated photoprotein Obelin作为RNA适体选择的靶标

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摘要

A variant of the Ca2+-regulated photoprotein obelin elongated with a hexahistidine peptide from the N-terminus was developed and studied. After immobilization on a metal-affine sorbent, the hybrid protein was applied as a target for the in vitro selection of RNA aptamers. According to the data of bioluminescent solid-phase microanalysis, the selection was shown to enrich the RNA library with obelin-affine molecules.
机译:开发并研究了用来自N-末端的六三氨基肽伸长的Ca2 + -Regulated光蛋白烯烃的变体。 在固定在金属染色体吸附剂上后,将杂交蛋白施用为用于体外选择RNA适体的靶标。 根据生物发光固相微量分析的数据,显示选择富集RNA文库与Obelin-achin分子。

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