Enhanced Activity of a Lipase by the Coating with a Quaternary Ammonium Alkyl-PEG Sulfate Ionic Liquid and Cooperative Activation with an Amino Acid

摘要

Three types of quaternary ammonium salts, tris(diethylamino)cyclopropenium cetyl-PEG10 sulfate (TAC1), N,N,N,N-tetraethylammonium cetyl-PEG10 sulfate (TEA), and N,N-diethylpiperidinium cetyl-PEG10 sulfate (PP₂₂), have been prepared and evaluated for their activation property of Burkholderia cepacia lipase using them as coating materials of the enzyme. Among the three tested IL-coated enzymes, the TAC1-coated lipase PS (TAC1-PS) was especially suitable for the transesterification of 1-(pyridin-2-yl)ethanol, 1-(pyridin-3-yl)ethanol, 1-(pyridin-4-yl)ethanol, and 4-phenylbut-3-en-2-ol. Although the reaction rate of 1-phenylethanol of the TAC1-PS-catalyzed reaction was inferior to that of IL1-PS, the enzyme was coated with the equivalent amount of l-methionine with TAC1, the resulting enzyme (TAC1-l-Met-PS) exhibited a higher activity compared to that of IL1-PS, and this was mainly contributed to the increasing K_cat value. We further succeeded in demonstrating the recyclable use of TAC1-PS in the [N_221MEM][Tf₂N] solvent system.]]>