LabVIEW-Based Software for Optical Stiffness Determination Using Boltzmann Statistics, Equipartition Theorem and Power Spectral Density Methods

摘要

An optical tweezers applications are becoming vast over time due to its unique ability to optically trap micron sized objects. However, calibration in term of optical stiffness determination for force-related experiments is essential. The optical stiffness calculation may take a dragfull amount of time. The aim of this study is to develop a user friendly and quick calculation LabVIEW based software for the determination of the optical stiffness of an optical tweezers. The determination employs three available methods: Equipartition Theorem (ET), Boltzmann Statistics (BS),and Power Spectral Density (PSD). The software requires only temporal trajectory of a trapped bead in an optical tweezers to calculate the optical stiffness and to evaluate the optical trap condition.