Assessment of the interplay between scaffold geometry, induced shear stresses, and cell proliferation within a packed bed perfusion bioreactor

Thibeaux Roman; Duval Hervé; Smaniotto Benjamin; Vennat Elsa; Néron David; David Bertrand

摘要

Abstract >By favoring cell proliferation and differentiation, perfusion bioreactors proved efficient at optimizing cell culture. The aim of this study was to quantify cell proliferation within a perfusion bioreactor and correlate it to the wall shear stress (WSS) distribution by combining 3‐D imaging and computational fluid dynamics simulations.NIH‐3T3 fibroblasts were cultured onto a scaffold model made of impermeable polyacetal spheres or Polydimethylsiloxane cubes. After 1, 2, and 3 weeks of culture, constructs were analyzed by micro‐computed tomography (μCT) and quantification of cell proliferation was assessed. After 3 weeks, the volume of cells was found four times higher in the stacking of spheres than in the stacking of cube.3D‐μCT reconstruction of bioreactors was used as input for the numerical simulations. Using a lattice‐Boltzmann method, we simulated the fluid flow within the bioreactors. We retrieved the WSS distribution (PDF) on the scaffolds surface at the beginning of cultivation and correlated this distribution to the local presence of cells after 3?weeks of cultivation. We found that the WSS distributions strongly differ between spheres and cubes even if the porosity and the specific wetted area of the stackings were very similar. The PDF is narrower and the mean WSS is lower for cubes (11?mPa) than for spheres (20?mPa). For the stacking of spheres, the relative occupancy of the surface sites by cells is maximal when WSS is greater than 20?mPa. For cubes, the relative occupancy is maximal when the WSS is lower than 10 mPa. The discrepancies between spheres and cubes are attributed to the more numerous sites in stacking of spheres that may induce 3‐D (multi‐layered) proliferation. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >通过有利于细胞增殖和分化，灌注生物反应器在优化细胞培养方面证明了有效。本研究的目的是在灌注生物反应器中量化细胞增殖，并通过组合三维成像和计算流体动力学模拟来将其与壁剪切应力（WSS）分布相关。地3T3成纤维细胞培养到由不透水的聚缩醛球或聚二甲基硅氧烷立方体。在培养1,2和3周之后，通过微计算断层扫描（μCT）分析构建体，并评估细胞增殖的定量。 3周后，在球形堆叠中发现细胞体积比在立方体3D-μCT重建的堆叠中，使用了生物反应器的重建作为数值模拟的输入。使用格子玻尔兹曼方法，我们模拟了生物反应器内的流体流动。在培养的开始，我们在支架表面上检索了在支架表面上的WSS分布（PDF），并在3个培养后与细胞的局部存在相关。我们发现，即使孔隙率和堆叠的特定湿润区域非常相似，WSS分布在球体和立方体之间也非常不同。 PDF较窄，平均WSS对于立方体（11→MPA）而不是用于球体（20≤MPa）。对于球形的堆叠，当WSS大于20≤MPa时，细胞通过细胞的相对占据最大值是最大的。对于多维数据集，当WSS低于10MPa时，相对占用是最大的。球体和立方体之间的差异归因于堆叠可能诱导3-D（多层）增殖的球体的差异。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第6期</span><b style="margin: 0 2px;">|</b><span>共13页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Thibeaux Roman&option=202" target="_blank" rel="nofollow">Thibeaux Roman;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Duval Hervé&option=202" target="_blank" rel="nofollow">Duval Hervé;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Smaniotto Benjamin&option=202" target="_blank" rel="nofollow">Smaniotto Benjamin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Vennat Elsa&option=202" target="_blank" rel="nofollow">Vennat Elsa;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Néron David&option=202" target="_blank" rel="nofollow">Néron David;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=David Bertrand&option=202" target="_blank" rel="nofollow">David Bertrand;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>MSSMat CentraleSupélecUniversité Paris Saclay CNRSGif sur Yvette France;</p> <p>LGPM CentraleSupélecUniversité Paris SaclayGif sur Yvette France;</p> <p>LMT ENS Paris‐Saclay CNRSUniversité Paris‐SaclayCachan France;</p> <p>MSSMat CentraleSupélecUniversité Paris Saclay CNRSGif sur Yvette France;</p> <p>LMT ENS Paris‐Saclay CNRSUniversité Paris‐SaclayCachan France;</p> <p>MSSMat CentraleSupélecUniversité Paris Saclay CNRSGif sur Yvette France;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cell proliferation&option=203" rel="nofollow">cell proliferation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lattice‐Boltzmann simulation&option=203" rel="nofollow">lattice‐Boltzmann simulation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=perfusion bioreactor&option=203" rel="nofollow">perfusion bioreactor;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=tissue engineering&option=203" rel="nofollow">tissue engineering;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=X‐ray micro‐tomography&option=203" rel="nofollow">X‐ray micro‐tomography;</a> </p> <div class="translation"> 机译：细胞增殖;格子-Boltzmann模拟;灌注生物反应器;组织工程;X射线微断层扫描; 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