Optimization of integrated chromatography sequences for purification of biopharmaceuticals

L?fgren Anton; Yamanee‐Nolin Mikael; Tallvod Simon; Fons Joaquín G.; Andersson Niklas; Nilsson Bernt

摘要

Abstract >With continued development of integrated and continuous downstream purification processes, tuning and optimization become increasingly complicated with additional parameters and codependent variables over the sequence. This article offers a novel perspective of nonlinear optimization of integrated sequences with regard to individual column sizes, flow rates, and scheduling. The problem setup itself is a versatile tool to be used in downstream design which is demonstrated in two case studies: a four‐column integrated sequence and a continuously loaded twin‐capture setup with five columns. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >继续开发集成和连续的下游净化过程，调整和优化随着序列的附加参数和依赖变量越来越复杂。本文提供了关于个体柱尺寸，流速和调度的集成序列的非线性优化的新颖视角。问题设置本身是在下游设计中使用的多功能工具，该工具在两个案例研究中演示：四列集成序列和具有五列的连续加载的双捕获设置。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第6期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=L?fgren Anton&option=202" target="_blank" rel="nofollow">L?fgren Anton;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yamanee‐Nolin Mikael&option=202" target="_blank" rel="nofollow">Yamanee‐Nolin Mikael;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tallvod Simon&option=202" target="_blank" rel="nofollow">Tallvod Simon;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fons Joaquín G.&option=202" target="_blank" rel="nofollow">Fons Joaquín G.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Andersson Niklas&option=202" target="_blank" rel="nofollow">Andersson Niklas;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nilsson Bernt&option=202" target="_blank" rel="nofollow">Nilsson Bernt;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Chemical EngineeringLund UniversityLund Sweden;</p> <p>Department of Chemical EngineeringLund UniversityLund Sweden;</p> <p>Department of Chemical EngineeringLund UniversityLund Sweden;</p> <p>Department of Chemical EngineeringLund UniversityLund Sweden;</p> <p>Department of Chemical EngineeringLund UniversityLund Sweden;</p> <p>Department of Chemical EngineeringLund UniversityLund Sweden;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chromatography&option=203" rel="nofollow">chromatography;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=continuous bioprocessing&option=203" rel="nofollow">continuous bioprocessing;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=integrated column sequences&option=203" rel="nofollow">integrated column sequences;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=multiple column design&option=203" rel="nofollow">multiple column design;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=optimization&option=203" rel="nofollow">optimization;</a> </p> <div class="translation"> 机译：色谱法;连续生物加工;集成柱序列;多列设计;优化; 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