Synthesis and Application of Streptavidin Functionalized Organosilica Microparticles

摘要

This article reports on the chemical synthesis, functionalization and application of streptavidin (SA)-coated organosilica microparticles. Thiol-functionalized organosilica microparticles are synthesized via a two-step approach involving acid-catalyzed hydrolysis and condensation of 3-mercaptopropyl trimethoxysilane (MPS), followed by base-catalyzed condensation. The surfaces of these particles are modified with 3-aminopropyltriethoxysilane (APS), and characterized by FTIR, XPS, and TGA. Then, APS-functionalized microparticles are covalently coated with SAs by using glutaraldehyde as a coupling agent, and characterized by FTIR, SEM, and AFM. Immobilization of biotinylated DNA onto microparticles via SA-biotin immuoreaction is confirmed by confocal microscopy. The results show that these SA-functionalized organosilica microparticles are useful carriers for DNA manipulation at the single molecule level. (c) 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41560.