Genetically Encoded Cyclopropene Directs Rapid, Photoclick-Chemistry-Mediated Protein Labeling in Mammalian Cells

摘要

The introduction of bioorthogonal organic reporters into proteins site-selectively through genetic, metabolic, or enzyme-catalyzed ligation methods in conjunction with a growing repertoire of bioorthogonal reactions, has allowed for the visualization and regulation of proteins in their native environment. Numerous small organic functional groups, such as ketones, azides, terminal alkynes, and terminal alkenes, as well as larger reactive bioorthogonal groups, such as cyclooctyne, norbornene, transcyclooctene, tetrazole, and tetrazine have been genetically encoded for site-selective protein labeling in vivo. To track fast protein dynamics in vivo, it is imperative that these genetically encoded bioorthogonal reporters direct fast and selective bioorthogonal labeling with the cognate biophysical probes, preferably with spatiotemporal control.