Development of Protein-Labeling Probes with a Redesigned Fluorogenic Switch Based on Intramolecular Association for No-Wash Live-Cell Imaging

摘要

Fluorescence labeling of proteins is a powerful technique for studying precise protein localization and movement in living cells. Currently, fluorescent proteins (FPs) are the primary tools in this field owing to their technical feasibility and convenience. Although various FPs have been reported, their properties such as protein size and brightness are not completely satisfactory for some biological applications. As an alternative to FPs, chemical methods utilizing synthetic fluorescence probes and fusion proteins have recently emerged.