A Fatigue-Resistant Photoswitclable Fluorescent Protein for Optical Naeoscopy

摘要

The function of biological macromolecules can be fully understood only within the context of their complex natural environment. Thus, recent years have witnessed an upsurge of studies aimed at exploring functional processes within living cells, tissues, and organisms. Fluorescence-based optical microscopy is a key method in this pursuit because it is minimally invasive and allows imaging in two or three dimensions over extended periods of time.