Conjugation of Transferrin to Azide-Modifled CdSe/ZnS Core-Shell Quantum Dots using Cyclooctyne Click Chemistry

摘要

Quantum dots (QDs) are semiconductor nanocrystals with unique optical properties that distinguish them from common organic fluorophores. For example, they give size-dependent emission spectra, high photoluminescence quantum yields, and large molar extinction coefficients, which gives the potential for single-molecule detection. They also have broad absorption cross-sections, which enables simultaneous excitation of mixed populations of QDs. Additionally, QDs are more resistant to chemical degradation and are less affected by photo-bleaching than conventional dyes.