Increasing the Efficacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study

摘要

The recent discovery of bioorthogonal click chemistry has created a new field in chemical biology in which biomolecules that are not directly encoded in the genome can be monitored in living systems. By hijacking a cell's biosynthetic machinery, a metabolic precursor functionalized with a bioorthogonal chemical tag is incorporated into target biomolecules, including glycans, lipids, proteins, and nucleic acids. Subsequently, a tailor-designed click reaction is employed to conjugate a complementary biophysical probe, which enables visualization or enrichment of the target biomolecules for molecular identification.