Microfluidic Multicolor Encoding of Microspheres with Nanoscopic Surface Complexity for Multiplex Immunoassays

摘要

Suspension arrays have emerged as a promising tool for biological screening and multiplex immunoassays owing to several advantages over conventional 2D planar arrays, such as faster binding kinetics, large surface areas, increased statistical power, and low assay cost.'1'21 Suspension arrays use identification tags on the microparticles rather than the spatial information employed in planar arrays. Two distinct approaches have been predominantly employed to write codes on particles in suspension arrays: spectrometric encoding and graphical encoding. In the first approach, the fluorescence spectra of polymeric microparticles are modulated by incorporating quantum dots or dye molecules with different emission wavelengths.