Photocontrol of Protein Activity Mediated by the Cleavage Reaction of a Split Intein

摘要

Photocontrol of proteins is an exquisite goal for the investigation of biological systems, for example to modulate conformational changes in proteins or to manipulate protein activity in living cells. Light can be applied with high temporal and spatial precision to biological samples and is furthermore orthogonal to most proteins and cells. However, there is no general blueprint of how to convert a non-photoresponsive protein of interest into a variant that can be specifically regulated by light.