Self-Assembled Arrays of Dendrimer-Gold-Nanoparticle Hybrids for Functional Cell Studies

摘要

Engineered surfaces with nanoscale features of gold on silicon or glass have recently been used to improve the understanding of adhesion-mediated environmental sensing of cells. Often such surfaces present a cell-binding ligand, such as arginine-glycine-aspartic acid (RGD) peptide motifs, at controlled intramolecular distances on an inert background surface such as polyethylene glycol (PEG). The adhesion mechanism of macromolecular ligands in which direct interaction with cells is nonspecific is not known and the cell response is dictated by the type and the concentration of proteins adsorbed from solution. Dendrimers may increase the availability and multivalency of cell-interacting ligands as a consequence of their branched shape and inherently high concentration of end groups. It is therefore interesting to examine the eventual effect of the macromolecular architecture on the cell viability by the controlled reduction of ligands on a surface. Herein, we demonstrate the fabrication of self-assembled macromolecular hybrid arrays in which the relative position of two anionic macromolecules of different architectures-a carboxy-functionalized dendrimer and a linear polymer-is straightforwardly controlled on a PEG surface. We also show how the interaction of primary human endothelial cells with these surfaces is modulated by the molecular spacing and how protein binding to the macromolecular arrays can be evaluated by using a standard surface plasmon resonance (SPR) technique.