Induced Nanoelectrospray Ionization for Matrix-Tolerant and High-Throughput Mass Spectrometry

摘要

Electrospray ionization (ESI) mass spectrometry is used extensively in biomolecular analysis, including proteomics, metabolomics, and glycomics. The newer nanoelectrospray method (nESI, flow rate < 1000 nLmin~(-1)) is derived from ESI, but uses a small-diameter spray tip that leads to improved ionization efficiency. Advantages of nESI over conventional ESI include 1) much improved ionization efficiency and ion transmission, 2) greatly reduced ion suppression and matrix effects, 3) longer analysis times that facilitate identification by tandem mass spectrometry (MS/MS), and 4) smaller sample volumes and lower absolute sample amounts, which improve compatibility with high-efficiency separation techniques, including capillary electrophoresis.