(Re)Solution of a Protein Fold Without Solution

摘要

The technique of mass spectrometry, now nearly 100 years old, was developed by Thomson and Aston at the Cavendish Laboratories, Cambridge University. These early pioneers used the gas-phase environment of their so called "mass spectrograph" as a laboratory for the study of atomic structure, unhindered by bulk interactions. Roll forward to 2010, and the gas phase remains a favorite location for detailed structure analysis, and particularly since the advent of electrospray ionization (ESI) there has been increasing applicability to biological macromolecules.