Photocontrol of Coiled-Coil Proteins in Living Cells

摘要

An ability to manipulate the activity of a specific protein inside living cells offers exciting prospects for the study of protein function in vivo. Photocontrol, in particular, offers the possibility of controlling protein function with very high temporal and spatial resolution that is not easily achieved by other methods. We aimed to develop an approach to the photocontrol of the coiled-coil protein structural motif inside living cells. Coiled coils in the form of basic leucine zipper (bZIP) domains are common in transcription factors that