Adhesion Effects of a Guanidinium Ion Appended Dendritic 'Molecular Glue' on the ATP-Driven Sliding Motion of Actomyosin

摘要

Biological systems sometimes utilize "adhesion" for performing required tasks. Typical examples include an amoeboid motion of white blood cells, which make use of carbohydrate moieties on their surface for the interaction with selectin. By using this adhesive interaction, selectin, which is produced at inflamed tissues, captures white blood cells from a rapid blood stream and allows them to operate site-selectively in wounded areas. Herein we report a water-soluble dendrimer that bears nine guanidinium ion pendants at its periphery (G1-(Gu~+)9R; Scheme 1), and serves as a synthetic inhibitor for the ATP-driven sliding motion of actomyosin by an adhesion mechanism (Figure 1). By using electrostatic and hydrogen-bonding interactions, the guanidinium ion can form a salt bridge with oxyanions, which exist ubiquitously on the protein surface.