Detection of Kinase Activity Using Versatile Fluorescence Quencher Probes

摘要

Protein phosphorylation is the most universal form of posttranslational modification of cell-signal transduction in living organisms. The human kinome comprises 518 protein kinases that control protein phosphorylation; irregular control of protein phosphorylation is a major cause of diseases such as cancer.Therefore, accurate probing of the kinase activity of a target protein is crucial for cancer diagnosis and high-throughput screening of anticancer drugs.