Self-Assembled TiO2 Nanocrystal Clusters for Selective Enrichment of Intact Phosphorylated Proteins

摘要

Mesoporous oxides have been used for selective separation and adsorption of biomolecules. Inorganic oxide materials such as silica can be synthesized into highly ordered mesoporous structures featuring high in-pore surface areas, narrow pore size distributions, adjustable pore sizes, and modifiable surface properties. Such unique properties favor the selective enrichment of proteins or peptides based on the size-exclusion mechanism. Classic mesoporous silica structures with narrow pore size distributions, such as MCM-41 and SBA-15, have been used to selectively enrich peptides from human plasma and exclude other proteins according to an accurate molecular weis ht (MW) cutoff.