Multiplexed Protein Arrays Enabled by Polymer Pen Lithography: Addressing the Inking Challenge

摘要

The ability to fabricate protein micro- and nanoarrays in a low-cost and high-throughput manner is important for a wide variety of applications, including drug screening, materials assembly, medical diagnostics, biosensors, and fundamental biological studies. Traditional approaches to making protein microarrays include photolithography and inkjet printing. Recently, studies also have focused on the miniaturization of protein patterns into the nanometer regime because high density protein arrays can provide increased detection sensitivity and, in principle, allow one to screen millions of biomarkers with one chip. Protein nanopatterns also can provide insight into important fundamental biological processes,'5' such as cell adhesion and differentiation.