RNA Interference:From Basic Research to Therapeutic Applications

摘要

Only ten years ago Andrew Fire and Craig Mello were able to show that double-stranded RNA molecules could inhibit the expression of homologous genes in eukaryotes.This process, termed RNA interference, has developed into a standard method of molecular biology.This Review provides an overview of the molecular processes involved,with a particular focus on the posttranscriptional inhibition of gene expression in mammalian cells, the possible applications in research, and the results of the first clinical studies.