Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes

摘要

Far-field fluorescence microscopy techniques with increased spatial resolution have the potential to enable a refined understanding of the intracellular organization of cells with near-molecular resolution. In this context, reversibly photo-switchable fluorophores arouse interest as key elements for optical nanoscopy. Nonetheless, labeling of intracellular structures with efficient reversibly photoswitchable fluorophores remains challenging. To date, either photoswitchable fluorescent proteins or pairs of fluorophores consisting of a reporter in close proximity to an activator are used.