Extending the Scope of Native Chemical Peptide Coupling

摘要

In the early 1990s,a total synthesis of native,functional proteins was considered an almost unachievable goal.However,thanks to Kent and co-workers' development of native chemical ligation,chemical protein synthesis has shifted into the realms of the achievable.The basis of this successful method is the chemoselective reaction of a peptide thioester with a cysteinyl peptide described by Wieland et al.This reaction takes place in aqueous buffer systems,and produces a "natural" peptide bond.The peptide segments can be coupled with one another in unprotected form.