Taking Fingerprints of DNA Polymerases: Multiplex Enzyme Profiling on DNA Arrays

摘要

DNA polymerases are used in a plethora of biotechnical applications, especially in the polymerase chain reaction (PCR), genetic cloning procedures, genome sequencing, and diagnostic methods.Highly processive and accurate DNA polymerases are desired for cloning procedures in order to give shorter extension times as well as more robust and high-yield amplification. A higher DNA polymerase fidelity may increase the reliability of genome sequencing and diagnostic systems.