Cell-Specific Gene Transfection from a Gene-Functionalized Poly(D,L-lactic acid) Substrate Fabricated by the Layer-by-Layer Assembly Technique

摘要

Controlled and efficient gene delivery/transfection is essential in many important biomedical applications. This process is highly regulated by the cellular microenvironment.Various strategies have previously been employed to deliver genes, but these achieved only limited success. However, this might be improved with gene delivery/transfection induced by a material's surface. For example, van den Beucken et al. demonstrated that a DNA-polymer layered film can be used for biomedical applications. Nevertheless, none of these reports established both cell-specific recognition and gene transfection on the surface of a material.