Fluorous Tags Catching on Microarrays

摘要

Microarrays provide a convenient way to probe biomolec-ular interactions with a minimum amount of sample and therefore have seen wide use in screens of primarily immobilized nucleic acids and proteins/peptides.Arrays of small molecules such as natural products,including carbohydrates,and druglike molecules have been slower to develop,in part because access is challenging to the compounds themselves with handles that allow specific attachment to an array surface.Most methods rely on the intrinsic reactivity of a nucleophilic group in the small molecule for covalent modification with reactive electrophiles on the microarray slide surface.Of course,multiple nucleophiles are common in small molecules and therefore the exact mode of attachment is unknown.A unique functional handle can be included in the initial design and execution of synthetic compound libraries,but this strategy adds complexity.