Synthesis of Protein Mimics with Nonlinear Backbone Topology by a Combined Recombinant,Enzymatic,and Chemical Synthesis Strategy

摘要

The development of powerful methods for protein synthesis provides new ways to understand protein function,either by regioselective incorporation of modified amino acids as physical probes into natural proteins or de novo protein design to mimic some of the structural and functional properties of native proteins.Expressed protein ligation and protein trans splicing have emerged as efficient semisynthetic approaches for the preparation of engineered proteins with normal linear backbone structure.