A Gold-Nanoparticle-Based Real-Time Colorimetric Screening Method for Endonuclease Activity and Inhibition

摘要

Nucleic acids act as the carriers of genetic information, and most organisms contain a type of enzymes called endonucleases, which can hydrolyze the phosphodiester linkages in the nucleic acid backbone. These nucleases are important for many processes involving the replication, repair, and recombination of nucleic acids. Endonucleases such as DNA gyrase and virus integrase play key roles in biological process such as the topological altering of DNA and the insertion of proviral DNA into host chromosomal DNA.